HPLC-Principle, Instrumentation, Parameters and Applications

HPLC-Principle, Instrumentation, Parameters and Applications

The contents of the Online Course are listed below:

1. Introduction to Author, Contents of HPLC [High performance liquid chromatography].

2. About HPLC.

3. Types of HPLC Techniques and Principle of HPLC.

4. Instrumental requirements – HPLC Pumps, different types of HPLC pumps.

5. Mixing Unit, gradient controller, solvent degassing.

6. Injectors and their types - Septum injectors, Stop flow injectors, Rheodyne injectors.

7. Columns and their types\- Guard Columns and Analytical Columns.

8. Types of Columns - Normal phase columns, Reverse phase columns, Ion exchange columns and Size exclusion columns

9. Detectors and their types.

10. Types of Detectors - UV/Visible detectors, RI detectors, fluorescence detectors, electrochemical detectors, conductivity detectors

11. Types of Detectors - evaporating light scattering detectors, mass detector, Ir detector and optical rotation detector

12. Recorders and Integrators.

13. Parameters used in HPLC, Retention Time, Retention Volume

14. Resolution and Separation factor

15. Theoretical Plates

16. Asymmetric Factor

17. Applications of HPLC

18. Quantitative Analysis

19. Multi-Component Analysis

About the Author:

Author has completed M. Pharmacy and pursuing Ph.D, completed Diploma in Nutrition and having 15 years of teaching experience in reputed Pharmacy Colleges. She has published 30 research papers in various International Journals and review Articles. She also published an Article in “Future of Education after Pandemic”

I have attended seminars and workshops in various reputed colleges and attended Indian Pharmacy Council (IPC).

Previous Courses of the Author are Introduction to Pharmaceutical Analysis and UV Visible Spectroscopy.

HPLC-Principle, Instrumentation, Parameters and Applications

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What you will learn

• Introduction to HPLC
• Types of HPLC
• Instrumental Requirements of HPLC

Rating: 5

Level: Intermediate Level

Duration: 1.5 hours

Instructor: Lakshmi Aswini