Gene Cloning Strategies
Gene Cloning Strategies
Part 1: Introduction
shot gun experiment explanation. Cosmid DNA. sonication. random fragments.
Part 2:
Applications, Human Genome project, Genome sequencing, whole Genome sequencing and genetic modification.
part 3:
Gene library
Genomic library
a) Nuclear Genomic library
b) Organelle Genomic library
Construction of Genomic library
Screening of genomic library
Diagram explanation
colony hybridization
plaque hybridization
application of genomic library
storage of genomic library
part 4:
CDNA library
Diagram and definition
construction of CDNA library
isolation mRNA
CDNAs into vector
suitable host
screening
part 5:
CDNA library continues and applications
Disadvantages and Genomic library vs CDNA library.
strategies:
Cancer, including genitourinary malignancy, is a consequence of accumulated genetic aberrations in genes involved in crucial regulatory pathways. The result is a deregulation of cellular behavior, leading to neoplastic transformation, uncontrolled cell proliferation and acquisition of metastatic ability. The development and perfection of techniques in the field of recombinant DNA technology, gene cloning, (differential) analysis of gene expression, and sequencing of genes and proteins have provided a wealth of information about the genetic aberrations associated with cancer development. This "recombinant DNA and gene cloning" technology and the recently developed DNA chip technology may provide new molecular diagnostic tools. Furthermore, the technology of gene cloning in combination with the progress in in vivo gene delivery techniques offers new treatment modalities, like gene therapy, additional to conventional therapies. This review is intended to provide a general introduction to the fundamentals or strategies of recombinant DNA and gene cloning techniques as a basis for understanding the rapidly expanding range of new diagnostic and therapeutic opportunities. Some illustrative examples are provided, addressing basic and biomedical research and possible clinical applications in genitourinary oncology.
Genomic library and CDNA library
Url: View Details
What you will learn
- Human Genome project
- Genome sequencing
- High throughput sequencing
Rating: 5
Level: All Levels
Duration: 2 hours
Instructor: Sowjanya Chollangi
Courses By: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
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