Thermodynamics and Protein Purification MCQ (Bio Chem)

Thermodynamics and Protein Purification MCQ (Bio Chem)

The thermodynamic free energy is a concept useful in the thermodynamics of chemical or thermal processes in engineering and science. The change in the free energy is the maximum amount of work that a thermodynamic system can perform in a process at constant temperature, and its sign indicates whether a process is thermodynamically favorable or forbidden. Since free energy usually contains potential energy, it is not absolute but depends on the choice of a zero point. Therefore, only relative free energy values, or changes in free energy, are physically meaningful

The free energy is a thermodynamic state function, like the internal energy, enthalpy, and entropy

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate

These questions will give you basic idea for Examination Preparation and/or interview on Thermodynamics and Free Energy and Protein Purification.

Please Note:

1. These questions are only for practice and understanding level of knowledge only. It is not necessary that these questions may or may not appear for examinations and/or interview questions

2. In this practice test, because of large amount of questions (around 14 questions) some of questions may have repeated

3. I had to put as 70% pass rate because there may also be wrong answers from my side

Examination Preparation and Interview questions on Thermodynamics and Protein Purification Practice (Bio Chemistry)

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What you will learn

• Thermodynamics and Free Energy
• Protein Purification

Rating: 4.125

Level: All Levels

Duration: 14 questions

Instructor: Manu Mitra